The use of immersion oil is essential to achieve the maximum numerical aperture of an oil immersion objective lens. The refractive index of the oil matches the glass cover slip and the lens, allowing more light to enter the lens and increasing resolution. Without immersion oil, the numerical aperture and resolving power of the microscope are reduced because of the lower refractive index of air compared to oil.
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This question's topic:
AAMA Certified Medical Assistant (CMA) /
Clinical Competency
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